abstract：:Rabbits hyperimmunized with fluorescyl-conjugated KLH exhibited bound ligand associated with a high affinity circulating IgG anti-fluorescein population. After cessation of immunogen administration the liganded complexes were eventually spontaneously cleared from the circulation. Individual rabbits synthesized autolog...

journal_title：Molecular immunology

authors： Voss EW Jr,Moore JK,Weidner-McGufficke KM,Denzin LK,Bedzyk WD,Voss VH

更新日期：1992-02-01 00:00:00

abstract：:Monoclonal antibodies to cyclosporine A (Cs), a potent immunosuppressant, were generated in BALB/c mice using a novel antigen prepared by linking Cs to a protein carrier via a photoactive cross-linking reagent, 4-benzoylbenzoic acid (BBa). Twenty-two monoclonal anti-Cs antibodies were generated, using Cs-BBa-bovine se...

journal_title：Molecular immunology

authors： Cacalano NA,Aggarwal R,Quesniaux VF,Cleveland WL,Erlanger BF

更新日期：1992-01-01 00:00:00

abstract：:Monoclonal antibodies (mAbs) to the hinge region of human IgG1 immunoglobulins were prepared by immunization with a proteolytic fragment of hinge segment coupled to keyhole limpet hemocyanin. A mAb, 4G3, was obtained capable of binding to intact IgG but not to partially reduced IgG. Using this mAb, inter-heavy (H) cha...

journal_title：Molecular immunology

authors： Azuma T,Takeda J,Motoyama N,Okada H

更新日期：1992-01-01 00:00:00

abstract：:To investigate the IL-6/IL-6 receptor system, we obtained five murine monoclonal antibodies against human IL-6, which neutralize its biological activity. We classified them into two groups (Type I mAb and Type II mAb) according to the epitopes they recognized. These two types of antibodies showed no difference in the ...

journal_title：Molecular immunology

authors： Shimamura T,Taki S,Honda H,Yokota M,Ito S,Takahara Y

更新日期：1991-11-01 00:00:00

abstract：:Monoclonal antibodies (MAbs) raised against trout immunoglobulin (Ig) recognize two independent Ig populations differing in the molecular weight of L chains. The trout Ig population recognized by MAb 2H9 represented congruent to 20% of the total Ig and contained L chains congruent to 26 kDa, whereas the trout Ig popul...

journal_title：Molecular immunology

authors： Sánchez C,Domínguez J

更新日期：1991-11-01 00:00:00

abstract：:The porcine complement proteins C3 and C4 have been isolated and then characterized using small-angle scattering methods. Within the limits of experimental errors, the porcine proteins are virtually identical with the corresponding human proteins as measured in terms of mol. wt, Mr and radius of gyration, R,: Mr(C3) =...

journal_title：Molecular immunology

authors： Osterberg R,Malmensten B,Boive T,Nilsson U,Stigbrand T,Mortensen K

更新日期：1991-09-01 00:00:00

abstract：:Peptides representing the amino-terminal sequence of protamines HP1 (sequence 1-12) and HP2 (sequence 1-11), the two major nuclear proteins of human sperm, have been synthesized. Rabbits were immunized either with peptide conjugated with a carrier or with free peptide. The resulting antisera were examined for their ca...

journal_title：Molecular immunology

authors： Rousseaux-Prevost R,Hublau P,Sautiere P,Rousseaux J

更新日期：1991-09-01 00:00:00

abstract：:The number of V alpha and V beta sequences of T cell receptors now available allows a meaningful analysis of their variability profiles. Variability plots were derived using a modified form of Wu and Kabat's algorithm: variability is not computed as a proportion of the number of different residues occurring at a posit...

journal_title：Molecular immunology

authors： Wuilmart C,Urbain J

更新日期：1991-09-01 00:00:00

abstract：:A porcine cDNA library was constructed using poly(A)+ RNA isolated from the spleen of an adult Minnesota miniature swine. Screening the library with antisera specific for porcine immunoglobulin light chains resulted in the selection and isolation of two recombinant clones, PLC18 and PLC3, which encode for kappa and la...

journal_title：Molecular immunology

authors： Lammers BM,Beaman KD,Kim YB

更新日期：1991-08-01 00:00:00

abstract：:The accuracy of calibrated immunoassays for measuring antibody concentration was analysed from a theoretical perspective. The study shows that there are theoretical limits on the accuracy of antibody immunoassays, which are determined by the affinity of the standard and unknown antibodies and the conditions chosen for...

journal_title：Molecular immunology

authors： Griswold WR,Chalquest RR

更新日期：1991-07-01 00:00:00

abstract：:mRNA encoding secreted immunoglobulin is synthesized either by termination of transcription 3' to secreted terminus sequences and 5' to the membrane terminus sequences or by cleavage of a pre-mRNA transcript containing both secreted and membrane sequences at the appropriate polyadenylation site 5' to the membrane sequ...

journal_title：Molecular immunology

authors： Weiss EA,Tucker PW,Finkelman FD,Yuan D

更新日期：1991-07-01 00:00:00

abstract：:Serotonin release from rat basophilic leukemia (RBL) cells, sensitized with a DNP-binding monoclonal IgE, was stimulated with solid surface (polystyrene)-bound DNP-amino acids. The stimulatory potency of DNP-amino acids was dependent on the structure of amino acid attached to DNP. Generally, DNP-amino acids with high ...

journal_title：Molecular immunology

authors： Varga JM,Kalchschmid G,Klein GF,Fritsch P

更新日期：1991-06-01 00:00:00

abstract：:Topoisomerase cDNA and various fragments thereof generated by the DNA polymerase chain reaction were cloned into plasmid expression vectors (pET series) and the expressed polypeptides were probed with scleroderma sera from seven different patients immunoreactive with topoisomerase I. All sera reacted selectively with ...

journal_title：Molecular immunology

authors： Piccinini G,Cardellini E,Reimer G,Arnett FC,Durban E

更新日期：1991-04-01 00:00:00

abstract：:Protein kinases have been implicated in a number of regulatory mechanisms including signal transduction in many cells. To address the possibility that the large granular lymphocyte (LGL) also uses one or more unique protein kinases for LGL functions, an efficient method was developed to obtain partial cDNA clones for ...

journal_title：Molecular immunology

authors： Yue CC

更新日期：1991-04-01 00:00:00

abstract：:The T-cell surface proteins CD4 (L3T4) and CD8 (Lyt2) are first expressed on thymocytes as they undergo maturation in the thymus. Two immature T-lymphoma cell clones SL12.4 and RS4.2 which constitutively express low or undetectable levels of CD4 and CD8 were used to investigate the activation of CD4 and CD8 gene expre...

journal_title：Molecular immunology

authors： Wilkinson MF,Fong AM,Huynh H,Hays EF,MacLeod CL

更新日期：1991-01-01 00:00:00

abstract：:A Brucella abortus protein with a molecular weight of 50 kDa has been shown to bind bovine immunoglobulin G from healthy, brucellosis-free animals. The Brucella immunoglobulin G binding molecule appears to be a protein, since it is susceptible to proteolysis. The protein is presumed to be located on the cell surface, ...

journal_title：Molecular immunology

authors： Bricker BJ,Tabatabai LB,Mayfield JE

更新日期：1991-01-01 00:00:00

abstract：:Four different proteases were screened for their capability of selectively digesting murine monoclonal IgGl to obtain active F(ab)2. For the screening, a series of five different mouse monoclonal antibodies (IgGl, k) was used, recognizing different tumor-associated antigens and currently used for radioimmunoimaging st...

journal_title：Molecular immunology

authors： Mariani M,Camagna M,Tarditi L,Seccamani E

更新日期：1991-01-01 00:00:00

abstract：:An expression cDNA library was constructed from the helper T cell hybridoma, A.1.1, which has been shown to produce constitutively proteins involved in the down regulation of the immune response. From this library we identified and characterized a cDNA clone, J6B7, by screening with a polyclonal antibody specific for ...

journal_title：Molecular immunology

authors： Lee C,Ghoshal K,Beaman KD

更新日期：1990-11-01 00:00:00

abstract：:The immunosuppressive cyclic undecapeptide cyclosporine (Cs) represents a useful model for studying the molecular basis of antibody-antigen interactions. The three-dimensional structure of the Cs molecule is known and a large panel of monoclonal antibodies (mAbs) to Cs has been well characterized by cross-reactivity s...

journal_title：Molecular immunology

authors： Schmitter D,Poch O,Zeder G,Heinrich GF,Kocher HP,Quesniaux VF,Van Regenmortel MH

更新日期：1990-10-01 00:00:00

abstract：:Artificial Salmonella serogroup B, D or Cl-specific glycolipids were prepared by covalently linking oligosaccharides corresponding to two O-antigen repeating units, obtained by phage enzyme hydrolysis of native O-antigenic polysaccharides, to octyl residues. Sheep erythrocytes coated with the artificial glycolipids we...

journal_title：Molecular immunology

authors： Grossman N,Svenson SB,Leive L,Lindberg AA

更新日期：1990-09-01 00:00:00

abstract：:T-cell-replacing factor (TRF)/IL-5 is a T-cell-derived glycoprotein which has pleiotropic activity on lymphoid and myeloid cells. IL-5 polypeptide translated into Xenopus oocytes are heterogeneous in molecular size (40,000 to 60,000 under nonreducing conditions) and yields a monomeric form (Mr of 25,000 to 30,000) und...

journal_title：Molecular immunology

authors： Takahashi T,Yamaguchi N,Mita S,Yamaguchi Y,Suda T,Tominaga A,Kikuchi Y,Miura Y,Takatsu K

更新日期：1990-09-01 00:00:00

abstract：:The primary structure of a human mu heavy chain (DAG) protein is described. The native protein is a circular decamer with a molecular weight (Mr) of 500 kDa, each decamer being constituted of the constant domains C mu 2, C mu 3 and C mu4 and interlinked by 15 disulfide bridges. At its NH2-terminal each monomeric chain...

journal_title：Molecular immunology

authors： Mihaesco C,Ferrara P,Guillemot JC,Congy N,Gendron MC,Roy JP,Sizaret PY,Mihaesco E

更新日期：1990-08-01 00:00:00

abstract：:Forty-eight group A streptococcal strains of different M types were screened for binding of human radiolabeled IgG. Three of the strains bound more than 80% of the added radioactivity and one of them, an M protein type 1 strain designated AP1, was selected for further analysis. Attempts were made to solubilize the IgG...

journal_title：Molecular immunology

authors： Akesson P,Cooney J,Kishimoto F,Björck L

更新日期：1990-06-01 00:00:00

abstract：:Antibodies that recognize Xenopus class II molecules have been developed. Mouse monoclonal antibodies were prepared by immunizing BALB/c mice with frog MHC antigens that had been partially purified with alloantisera, and by immunizing mouse spleen cells in vitro with activated Xenopus T lymphocytes. In addition, five ...

journal_title：Molecular immunology

authors： Flajnik MF,Ferrone S,Cohen N,Du Pasquier L

更新日期：1990-05-01 00:00:00

abstract：:The antigen-limiting nature of microtiter ELISAs predicts that antibodies of minor classes may be underestimated when the same specimen contains large amounts of IgG antibodies specific for the same antigen. Such competitive inhibition can be diagnosed from ELISA titration plots. A method is described to eliminate the...

journal_title：Molecular immunology

authors： Butler JE,Heo Y,Adams P,Richerson HB

更新日期：1990-04-01 00:00:00

abstract：:Conjugates of monoclonal antibodies and Pseudomonas exotoxin A (PE) were formed with disulfide or thioether bonds. Thioether conjugates which formed with succinimidyl 4-(N-maleimidomethyl)-cyclohexane-1-carboxylate (SMCC) modified PE and reduced antibody formed with an 80% yield of equimolar conjugate within 30 min wi...

journal_title：Molecular immunology

authors： Morgan AC Jr,Sivam G,Beaumier P,McIntyre R,Bjorn M,Abrams PG

更新日期：1990-03-01 00:00:00

abstract：:A two-step purification procedure of Par j I from the whole Parietaria judaica pollen extract is described. The first step consisted of gel filtration HPLC using a TSKG 3000 SW column, and 0.1% trifluoroacetic acid as the eluant. By this method, proteins were separated from the highly colored material present in the e...

journal_title：Molecular immunology

authors： Polo F,Ayuso R,Carreira J

更新日期：1990-02-01 00:00:00

abstract：:Notexin and nigexine are monomeric phospholipases A2(PLA2s) from the venoms of Notechis scutatus scutatus and Naja nigricollis, respectively. Polyclonal antibodies raised in mice against these antigenic proteins displayed non-reciprocal cross-reactivity; anti-notexin antibodies recognized notexin but not nigexine, whe...

journal_title：Molecular immunology

authors： Mollier P,Chwetzoff S,Ménez A

更新日期：1990-01-01 00:00:00

abstract：:1,25-Dihydroxyvitamin D3 (1,25-D3) is known to have potent inhibitory effects on human peripheral blood mononuclear cell (PBMC) functions. Previous experiments suggest that addition of interleukin-2 (IL-2) to cell cultures can reverse the antiproliferative action of 1,25-D3. Previous studies have also shown that the C...

journal_title：Molecular immunology

authors： Jordan SC,Lemire JM,Sakai RS,Toyoda M,Adams JS

更新日期：1990-01-01 00:00:00

abstract：:Purified human C5 was converted non-enzymically to an activated form as defined by its ability to participate in reactive lysis. This conversion occurred following exposure to systems that generate oxygen radicals, namely addition of H2O2 in the presence of ascorbic acid and iron or the addition of xanthine oxidase, a...

journal_title：Molecular immunology

authors： Vogt W,Damerau B,von Zabern I,Nolte R,Brunahl D

更新日期：1989-12-01 00:00:00

abstract：:Activation of Jurkat T cells with phytohemagglutinin, CD3 or CD2 mAbs results in a marked inhibition of phosphatidylserine (PS) synthesis. Monitoring PS synthesis in T cells shows that: (i) after modulation of CD3 molecules the cells become refractory to further treatment with CD3 mAbs as well as to a further challeng...

journal_title：Molecular immunology

authors： Pelassy C,Dallanegra A,Aussel C,Fehlmann M

更新日期：1989-11-01 00:00:00

abstract：:The immunoglobulin JHC mu intron was cloned from genomic DNA of a VHa3 rabbit and a 1257 bp sequence which contains conserved enhancer and splice sites was determined. From positions 315 to 1257, there is approximately 72 and 67% similarity to available sequences of man and mouse, respectively (counting gaps as single...

journal_title：Molecular immunology

authors： Mage RG,Newman BA,Harindranath N,Bernstein KE,Becker RS,Knight KL

更新日期：1989-10-01 00:00:00

abstract：:A recent report indicated that T200 molecules interact with elements of the cytoskeleton in BW5147 T lymphoma cells. We have confirmed the cytoskeletal association of T200 by examining nonionic detergent-soluble and detergent-insoluble fractions of murine T cell tumor cell lines, cloned cytotoxic T lymphocyte lines, a...

journal_title：Molecular immunology

authors： Taffs RE,Ewald SJ

更新日期：1989-10-01 00:00:00

abstract：:Extracts of blood lymphocytes, polymorphonuclear neutrophils and B, T or monocytic cell lines were analyzed by two-dimensional gel electrophoresis and immunoradiometric assay after electro-transfer to nitrocellulose sheets with radiolabelled polyclonal or monoclonal antibodies specific for beta 2-microglobulin. Four d...

journal_title：Molecular immunology

authors： Vincent C,Ramackers JM,Bonnefoy N,Revillard JP

更新日期：1989-08-01 00:00:00

abstract：:Hybridomas producing anti-histone monoclonal antibodies were generated from a 2-month old MRL lpr/lpr mouse. Five IgM antibodies showed binding to histone H3 and three of these IgM antibodies also bound histone H1. Two IgG2a antibodies (MRA3 and MRA12) were specific for histone H1 and their binding was further charact...

journal_title：Molecular immunology

authors： Monestier M,Fasy TM,Böhm L

更新日期：1989-08-01 00:00:00

abstract：:We have developed two distinct solid-phase immunoassays for the detection of antigen binding activity by products of antigen binding T cell hybridomas in the absence of MHC. Two suppressor T cell hybridomas studied (34s-18 and 34s-704) are specific for keyhole limpet hemocyanin, a protein antigen, and the other suppre...

journal_title：Molecular immunology

authors： Bristow CL,Marchalonis JJ

更新日期：1989-07-01 00:00:00

abstract：:Vasoactive intestinal peptide (VIP) induces phosphorylation of a basic 38,000 mol. wt protein in a human lymphoblastic cell line (Molt 4b) and a human colon carcinoma cell line (HT29). In both cell types, VIP interacts with specific high affinity receptors to activate adenylate cyclase and cAMP-dependent protein kinas...

journal_title：Molecular immunology

authors： O'Dorisio MS,Campolito LB

更新日期：1989-06-01 00:00:00

abstract：:Five murine A/J hybridomas (the 35-20 group) produce anti-digoxin antibodies that have homologous heavy and light chain immunoglobulin variable regions (VH and VL), yet differ from each other in fine specificity and affinity for digoxin and related cardiac glycosides [Mudgett-Hunter et al. Molec. Immun. 22, 477-488 (1...

journal_title：Molecular immunology

authors： Near RI,Haber E

更新日期：1989-04-01 00:00:00

abstract：:The capsid protein of hepatitis B virus (P19) is made of 183 amino acids and carries the antigenic sites of hepatitis B core antigen (HBcAg) and hepatitis B e antigen (HBeAg) on the amino-terminal domain. The carboxyl-terminal domain of P19 (amino acids 150-183) is arginine-rich (47%) and faces the interior of the nuc...

journal_title：Molecular immunology

authors： Machida A,Ohnuma H,Takai E,Tsuda F,Tanaka T,Naito M,Munekata E,Miyakawa Y,Mayumi M

更新日期：1989-04-01 00:00:00

abstract：:alpha 2-Macroglobulin (alpha 2M) complexed with proteinases or modified by the action of amines has been shown to affect immune responses in vitro, though as yet the mechanisms are poorly understood. Supernates from rabbit lymphoid cells cultured in medium with normal rabbit serum and 35S-methionine (or 14C-leucine) w...

journal_title：Molecular immunology

authors： Schlesinger C,McEntire J,Wallman J,Skosey JL,Hanly WC,Teodorescu M

更新日期：1989-03-01 00:00:00